中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (29): 5389-5395.doi: 10.3969/j.issn. 2095-4344.2012.29. 015

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

磷酸钙骨水泥-淫羊藿复合材料促进骨质疏松大鼠骨折的愈合

尹 宏，钱卫庆   

  1. 南京中医药大学附属第三临床学院，南京市中医院骨伤科，江苏省南京市 210001
  • 收稿日期:2011-11-02 修回日期:2011-12-18 出版日期:2012-07-15 发布日期:2012-07-15
  • 通讯作者: 尹宏，主任医师，南京中医药大学附属第三临床学院，南京市中医院骨伤科，江苏省南京市 210001
  • 作者简介:尹宏，男，1960年生，江苏省南京市人，汉族，1984年南京医科大学毕业，主任医师，主要从事骨关节炎及骨质疏松性骨折研究。 hongy6011@ 163.com

Calcium phosphate cement/epimedium composite materials for improving fracture healing of osteoporotic rats

Yin Hong, Qian Wei-qing   

  1. Department of Orthopedics and Traumatology, Nanjing Hospital of Traditional Chinese Medicine, the Third Clinical Affiliated Hospital, Nanjing University of Chinese Medicine, Nanjing 210001, Jiangsu Province, China
  • Received:2011-11-02 Revised:2011-12-18 Online:2012-07-15 Published:2012-07-15
  • Contact: Yin Hong, Chief physician, Department of Orthopedics and Traumatology, Nanjing Hospital of Traditional Chinese Medicine, the Third Clinical Affiliated Hospital, Nanjing University of Chinese Medicine, Nanjing 210001, Jiangsu Province, China hongy6011@ 163.com
  • About author:Yin Hong, Chief physician, Department of Orthopedics and Traumatology, Nanjing Hospital of Traditional Chinese Medicine, the Third Clinical Affiliated Hospital, Nanjing University of Chinese Medicine, Nanjing 210001, Jiangsu Province, China hongy6011@ 163.com

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543

被引次数

3

摘要：

背景：淫羊藿具有促进成骨细胞增殖分化的作用。
目的：观察磷酸钙骨水泥与淫羊藿复合材料对骨质疏松大鼠骨折愈合的影响。
方法：将SD大鼠随机分组：实验组切除双侧卵巢建立骨质疏松动物模型，对照组仅切除卵巢周围少许脂肪组织。实验组将造模成功动物制作右侧股骨远端松质骨骨折，随机分3组治疗：克氏针内固定组、磷酸钙骨水泥组、磷酸钙骨水泥-淫羊藿复合物组。
结果与结论：①碱性磷酸酶活性：治疗后2，4，8周时，3组均有不同程度升高，磷酸钙骨水泥-淫羊藿复合物组最高(P < 0.05)。12周时，磷酸钙骨水泥-淫羊藿复合物组基本恢复至正常范围，其他两组虽有所下降，但仍维持在一个相对较高水平。②生物力学检测：磷酸钙骨水泥-淫羊藿复合物组骨折段承受的最大载荷明显高于其他两组(P < 0.05)，且增加幅度最大。③组织学检查：磷酸钙骨水泥-淫羊藿复合物组纤维骨痂及骨样骨痂出现较其他两组早，且数量较多，骨愈合质量明显高于其他组。表明磷酸钙骨水泥-淫羊藿复合材料具有较好的骨诱导活性，可有效促进骨质疏松大鼠骨折的愈合。

关键词: 淫羊藿, 磷酸钙骨水泥, 大鼠, 骨质疏松症, 骨折愈合, 生物材料

Abstract:

BACKGROUND: Epimedium can promote the proliferation and differentiation of osteoblasts.
OBJECTIVE: To observe the effects of calcium phosphate cement (CPC)/epimedium composite materials on fracture healing of osteoporotic rats.
METHODS: Sprague Dawley rats were randomly divided into control group and experimental group. Ovariectomy-induced osteoporosis rat models were established in the experimental group, while surgical excision of a small amount of fatty tissue around the ovaries was performed in the control group. Cancellous bone fracture was prepared in the distal end of the right femur of rats in the experimental group, and the rats were subdivided into three groups for treatment: Kirschner wire group, CPC group and CPC/epimedium group.
RESULTS AND CONCLUSION: Alkaline phosphatase activity: increasing in varying degrees of alkaline phosphatase activity could be seen in the three groups at 2, 4 and 8 weeks after treatment, and the improvement in alkaline phosphatase activity of the CPC/epimedium group was the highest (P < 0.05). At 12 weeks, alkaline phosphatase activity of the CPC/epimedium group returned to normal, and those of the other two groups maintained a high level despite some decrease. Biomechanical detection: the maximum load of the CPC/epimedium group was obviously higher than those of the two other groups (P < 0.05), and the rate of increase in the maximum load of the CPC/epimedium group was the highest. Histological examination: fibrous osteotylus and bone callus of the CPC/epimedium group appeared more early, and both the quantity and the quality of new bone were superior to those of the other groups. It is indicated that the CPC/epimedium artificial bone possesses a good osteoinductive activity, and can improve the fracture healing of osteoporotic rats.

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